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Elutes were resolved on SDS PAGE and analyzed by immunoblotting with mouse anti-ABCA1 (1:500 dilution), rabbit anti-LXRα (Abcam, ab3585, 1:1000 dilution) and rabbit anti-GIV antibody (ABT80, Millipore sigma, Burlington, MA, 1:500 dilution) Single and sequential DuoLink® Proximity Ligation Assay (PLA) PMA-differentiated THP-1 wild-type (WT) cells were seeded on sterile glass coverslips at a density sufficient to reach ∼60–70% confluency and treated with 25 nM phorbol 12-myristate 13-acetate (PMA

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Acta 1822 , 1374–1386 (2012)